FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_6751.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_6751.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23276018
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT278330.11957801373069914No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC241180.10361737991438226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC125600.030.0134544
AGTCTAA138000.027.6842563
GAGTCTA160850.024.0219572
TCTAACA169350.022.1313345
GTTACGA44300.019.3099234
AGAAGCG138750.017.346324
TGCGCGA172700.017.000599
TACGACT54600.016.1983016
GAAGCGA149750.015.9753125
GAACGTA130850.015.5680735
GTAGCAT108150.015.5511927
TAGCATA108900.015.3774888
AACGTAT133500.015.1504376
CGAACGC215550.015.1359171
GTGCGCG198750.015.06413658
TTACGAC59250.015.0493935
GAGCTCG156550.014.2163018
TGTTACG66700.013.5858083
ATCCCGA342300.013.1730314
ATGACGT115400.013.1293347
Produced by FastQC (version 0.11.5)