FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_6215.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_6215.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19671731
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAAACCAAGTGATCTACCCTTGGTCAGGTTGAAGGTTAGGTAAAACT378010.19215899200736325No Hit
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC352820.17935381487272267No Hit
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC339420.17254200964826125No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC204310.10385969592609821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT98050.033.1266861
CGACCTA36300.032.9517564
TGATCCT125900.032.3026664
GATCCTG138150.030.435355
TACGACT104100.030.2232632
CACTATA94400.029.258579
ATAATAC77250.028.52843
CTATTAG65450.028.3550518
TGTACTC119350.027.8799862
ACTCACT107250.026.9021516
AACGTAT459900.026.355676
GCTCAGA88400.025.6680765
GAACGTA483450.025.176795
CGTATTC490450.025.1721348
CCGGGAA445150.025.1471331
GTACTCT135900.024.7514613
TTGATCC168250.024.5596473
TCAGATT86250.024.2907582
TCTAGTT138400.024.147167
GTATTCA512050.024.1244459
Produced by FastQC (version 0.11.5)