FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_6119.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_6119.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23529509
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC354340.1505938776708005No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA310230.13184720514142476No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG294630.12521723253978653No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC282020.11985800468679562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTGT441700.032.62845145
AGCAATT81900.032.4854357
CATTGTA287900.031.878618145
CAATTAG96800.031.6041139
TACTACT56750.031.4250346
CTATTAG65550.029.9711288
CGATCTA43400.029.398794
CTTACAT61950.028.205571
AACGTAT687500.028.1226866
CCGGGAA584500.027.9470391
GCTCAGA104400.027.6368855
GTATTCA735350.026.3025729
GGAACGT664500.025.8340934
GATCCTG162100.025.804735
CGGGAAC646650.025.605562
GAGCAAT111750.025.3001796
AATACTA85450.025.112284
ACGTATT789700.024.8044557
TTGAACG109350.024.4632437
CGTATTC795500.024.2864238
Produced by FastQC (version 0.11.5)