FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_5867.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_5867.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21430688
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC274600.1281340104433418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATTA45650.045.576717145
GATCCTG95450.033.1139875
CTATTAG95500.026.4164688
CCGGGAA442850.025.114141
TTTGATC185400.024.6730352
AACGTAT495350.024.5279796
TCAGATT126200.024.2413062
GATCATG64850.023.5870385
CCATTGT369350.023.4548145
CAATTAG73900.023.2489229
TGGATGC156950.022.5402853
CTGGCTC161800.022.357389
TTGATCA72700.022.2367533
GAACGTA557500.021.9106865
GGCTCAG139500.021.6182254
CATTGTA229100.021.5172145
TATTAGT124900.021.4752489
ACGTATT586600.021.13267
CGTATTC605100.020.8339358
AGAAATG233200.020.392817145
Produced by FastQC (version 0.11.5)