FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_4340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_4340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19673317
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA26100.032.497534
GATCCTG113850.025.7885535
TGATCCT119550.024.8621854
AACGTAT400800.024.436145
CAATTAG89350.023.9348879
CCGGGAA340250.023.694771
TCAGATT99000.022.7735792
CGTATTC429500.022.7526387
TATTCAC448250.022.0110999
GATTGAA152850.021.9594025
GTATTCA453600.021.4638128
TCACGAG75800.021.3275892
CATTGTA256800.021.11605145
CCATTGT362900.021.015265145
CTGGCTC149900.020.8922889
GGAACGT397050.020.7414044
GAACGTA493000.020.6307834
TTGAACG97350.020.6276057
CGGGAAC403900.020.3556541
GGGAACG415250.019.98952
Produced by FastQC (version 0.11.5)