FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_4340.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_4340.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24274672
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA104700.029.7033422
GTTACGA66800.027.1305874
CTTACAT118950.026.6961251
TTACATT117600.026.691642
TCTAACA121150.026.4481015
AGTCTAA124600.024.9593933
TAGCATA102750.024.9051138
CAATTAG59400.024.896569
AACGTAT481050.024.3978886
TTACGAC75500.023.9082625
GAACGTA507200.023.3972645
TGCCAGT115450.023.3584372
GCTCAGA106800.023.146155
GTAGCAT110800.023.0302437
CTCGGCT102950.022.8873541
CGGGAAC478350.022.5957412
CCATTGT345600.022.591345145
GATCCTG164500.022.519085
ACGTATT531550.022.4891057
ACATTCA141300.022.266054
Produced by FastQC (version 0.11.5)