FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_3256.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_3256.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736709
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT289090.1464732544822949No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC262260.13287929613797317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC81350.041.2591633
TAGGGAA112200.039.4807131
TAATACG85100.039.3557624
ATACGAC135300.035.633661
CACTATA138900.035.385259
ACGACTC145700.033.982723
GTTACTA75650.033.5394633
TCTAGTT131300.033.5135087
TACGACT148400.033.4621352
GACTCAC149100.032.964615
CGACTCA155000.031.5695194
ACTCACT158500.031.466986
GTACTCT143050.030.9635223
TAGTTGT145300.030.8830269
AATAATA108900.030.6881032
CTGTTAC84000.030.6397821
TACTCTA146050.030.5756034
TGTTACT83050.030.5509992
TAGCATA40150.030.333228
TCACTAT164750.029.8331478
Produced by FastQC (version 0.11.5)