FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_3256.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_3256.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23905421
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA361520.151229296484676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG116000.029.8725935
GCTCAGA129700.026.5495195
CAATTAG88900.023.077499
TCCTGGC157850.022.1822477
CGTGCGT76450.021.9047558
GAAACTA176250.021.470614
TGATCCT160550.021.3576684
CTATTAG107300.021.1469888
CTCGGCT112600.019.7671411
TCCAGTA116050.019.73998145
CTAACCA185000.019.5930588
CCTGGCT176200.019.1315848
CATTGTA220450.018.84297145
TTGAACG125200.018.818377
CGAACGC383250.018.4255581
ACGAGCA73300.018.3955154
AACGTAT387350.018.2288116
ATTGAAC136150.018.2100646
TGTCCTA109300.018.1732855
CCATTGT296500.018.019703145
Produced by FastQC (version 0.11.5)