FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30982.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30982.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20541903
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA1079250.5253894928819399No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA853360.41542402376255017No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG558260.2717664473442407No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC433500.211032054819848No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC394000.19180306712576728No Hit
GTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCACAGA370010.18012449966295724No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCG366030.17818699659909795No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG347620.1692248279042112No Hit
AAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGC293900.14307340464026144No Hit
TGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTA291730.14201702734162458No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC284590.13854120526223887No Hit
ATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTA282430.1374896960617524No Hit
CGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGG281800.1371830058782772No Hit
CGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGT270490.1316771868701746No Hit
GTCCCGCCCTACTTTTCGTACGCTTAGTACCACTGTTGAGATTTCGAATA267630.13028491079916013No Hit
CCCGCCCTACTTTTCGTACGCTTAGTACCACTGTTGAGATTTCGAATACG265080.12904354577080807No Hit
CGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGATTGCGAACC226230.11013098445650338No Hit
CTGGTCTCGAACGACTCTTTAGTGTGGTTAAACCACAAGGGAAGTCTCAT225430.10974153660447136No Hit
CACCAACTAGCTAATCTGCCATCGGCCGCTCCAATAGCGCGAGGTCCGAA220850.10751194765158809No Hit
CCCATATTCAGACAGGATTTCACGTGTCCCGCCCTACTTTTCGTACGCTT216040.10517039244124558No Hit
CCCGAAACCAGGTGATCTATCCATGGTCAGGATGAAGGTTGGGTAACACC211070.1027509476604967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA205300.064.6200564
TCACGAG204350.062.8630332
CACATTA39950.056.616222145
AGCAATT258850.055.536397
GAGCAAT288750.052.47216
TTACATT168900.051.1626322
CAATTAG306700.050.2281539
CTTACAT172800.049.8456841
GCAATTA309700.049.5777478
CGAGCAA303250.047.2618875
TTCAGTA197600.043.8413667
TACATTC206900.041.8709343
GGTCACA53050.041.2739871
CCGGGAA460000.040.9168051
TGTCCTA133850.040.4582825
GCTCAGA123150.039.440795
GGTTAGA513250.039.2805184
GGCTCAG140600.038.2065734
CTGGCTC138000.036.7201422
TTTCCGT344150.035.599346145
Produced by FastQC (version 0.11.5)