FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30887.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30887.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20347466
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT506870.24910718612332367No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA445700.21904447462892923TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT441160.21681323856248244TruSeq Adapter, Index 7 (97% over 38bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG378030.18578726215834446No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG363880.17883307926402234No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC318380.1564715724306899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC112000.056.960053
ATACGAC191250.047.2352371
TAGGGAA143750.038.281011
CACTATA243700.037.481749
GACATAC64400.037.260349
TAATACG177750.036.665354
TACGACT264950.035.2422032
CGTCTTG143400.034.073647145
CGACTCA293500.031.8630494
CTCACTA300400.030.3347977
GAGCATT218400.029.542226
AACAGAC82500.028.734235
ACTCACT327050.028.4614226
ACGACTC329300.027.7386023
TCCAGTA97700.026.860981145
AATACGA243500.026.7054085
GACTCAC362450.026.1816645
TCACTAT361200.026.1116438
TCTAGGC33600.024.8121457
CGGATGT72200.024.803291
Produced by FastQC (version 0.11.5)