FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30848.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30848.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24600110
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG1075200.4370712163482196No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC925480.37620969987532576No Hit
CACACGTGCTACAATGGTTGGTACAACGAGTTGCGAGTCGGTGACGGCAA347620.1413083112229986No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC282930.11501168084207752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA401650.040.881203145
CTATTAG115050.038.562758
CCGGGAA676300.034.6923141
CGATCTA67450.029.8791854
AACGTAT909650.029.503125
GAACGTA927850.029.244754
GGGAACG935350.028.8941152
GGAACGT933950.028.4250663
CGGGAAC939250.028.1294541
TTAGAGT224800.028.023896
TATTCAC968850.027.6702829
CCATTGT641400.027.487665145
ACGTATT989750.027.3644286
CGTATTC1042100.025.6210217
GTATTCA1049700.025.559788
GTTAGAG264350.023.584415
GGTTAGA294050.022.7555034
TATTAGT204950.022.4609979
CGAGCTA85950.022.1827684
GGGTTAG301800.021.4745983
Produced by FastQC (version 0.11.5)