FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30785.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30785.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22697381
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT444820.19597855805478173No Hit
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC429550.18925090960935095No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG421010.18548836096992863No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA341810.1505944672647474No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGAACTCT339550.14959875767164502No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT312480.13767227152771502No Hit
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT290540.12800595804423426No Hit
CGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGTC267250.11774486228168793No Hit
GCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGT257310.11336550238990128No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG249150.10977037394754928No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA245620.10821512843265926No Hit
AGCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACT237680.10471692747282164No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC231660.1020646390876551No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC230220.10143020465665178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAC152050.046.533844145
CTTACAT87200.042.4029051
CCATAGC83150.038.710534
GGCCATA89400.037.544982
CACATTA95150.037.485374145
TTGGTCC192150.035.766496
TTACATT104600.035.4158482
TACATTC106600.035.2953573
CCACCAT300000.034.7492183
CCATGCC387100.034.2717556
ACCATGC330100.033.996475
GTCCCAC215600.032.6157659
ATTCAGT131850.032.4399576
GAGTTGG214300.032.204923
ACTCGAA92100.032.1150745
ATATCGC238350.031.479924
TGCGTGT129000.030.6839542
GAGTCTA50850.030.6517942
TAGACCT106850.030.5991577
GTGATAT247150.030.3613911
Produced by FastQC (version 0.11.5)