FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30782.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30782.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065015
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG639900.39831895581796845No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG385540.23998732649798335No Hit
CCCACGAGTGCCGTTTAGGCTGAATCCGCTTGAACCTTGCTGACAAGGCG344310.21432286244363916No Hit
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC274630.17094910897997917No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGGACTCT238620.14853394161163247No Hit
GCCTTTCACCCCTATCCACAGCTCATCCCCGCATTTTGCAACATGCGTGG224470.13972598220418717No Hit
CGCGAAGGCATATATTCCTTTGAACCAATACGTTCGCTTTAGGTTACCGG205210.12773719787998952No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT185900.11571729002431681TruSeq Adapter, Index 7 (97% over 38bp)
CGACTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAA174640.10870827073613065No Hit
GTTCGCGAAGGCATATATTCCTTTGAACCAATACGTTCGCTTTAGGTTAC173000.10768741890374829No Hit
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT171300.10662921883359586No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA165910.10327410214058313TruSeq Adapter, Index 7 (97% over 38bp)
ACGACTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAA163530.1017926220423697No Hit
GTGCGTGTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCG162020.10085269139182254No Hit
CCGGCGATGACCTACTCTCCCACACCCACAAAAGTGCAGTACCATCGGCG161080.10026756900009118No Hit
CCACGAGTGCCGTTTAGGCTGAATCCGCTTGAACCTTGCTGACAAGGCGG161070.10026134429379618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT161250.062.3635251
TTACATT170900.059.0894932
TACATTC172450.058.810613
TTCAGTA180500.055.705637
ACATTCA182850.055.425814
ATTCAGT195300.051.892526
CATTCAG199150.051.107745
TCAGTAG199350.050.692818
CAGTAGC217000.046.6030469
ACGAGTG122300.046.1754234
TACGACT103700.042.7133522
CACGAGT132200.042.2789463
TGTCCTA127800.038.685965
CGAGTGC157400.038.181215
ATATATG91850.037.805607145
GAGTGCC166650.037.540966
ATCACAC144800.037.498436145
GTGATAT182950.036.8556821
AGTGCCG170800.036.3317037
GGCATAT98300.035.9149557
Produced by FastQC (version 0.11.5)