FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_30782.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_30782.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814874
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT85550.014.5751482
GTACTCT95350.014.14154153
CATTATG138300.013.995784145
ACTCTAG111200.013.8860685
ACACGTA55200.013.658482145
CTCTAGT104200.013.4970396
TAATACG70700.013.3299474
TACTCTA105250.013.293514
GACTCAC108700.012.6048235
CACTATA94750.012.3948229
CTAGTTG112450.012.3778818
ATACGAC84500.012.3554981
ACGACTC99400.012.2525763
CGACTCA101550.012.207334
ACTCACT130050.010.8699816
TGTACTC123450.010.6289892
TAGTTGT133100.010.2940979
CTCACTA113000.010.2646957
TCTAGTT149500.010.134677
GTATGCC370600.010.054625145
Produced by FastQC (version 0.11.5)