FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_2397.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_2397.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21540450
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACTACAGCTTAGCATTGACCTTGGTCATCCGATGTGTAGGATAGGC240010.11142292756186616No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGAACTCT228300.10598664373306964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA73500.050.005894
ATCTATT100450.040.9198156
TTCAGTA80850.036.7624447
CTATTAG141050.034.3837788
CTTACAT94700.031.158841
TGCCGTA226050.030.2418089
TTACATT100850.029.8314782
GTGATAT165300.029.4297471
ATTCAGT115050.028.228776
TCTATTA148300.027.716767
TGTCCTA83250.027.3429855
ATTAGAA66800.027.1308926
CCATGCC254700.026.8685116
AACGTAT465200.026.5696135
CTGGCTC181400.026.0561899
GTATTCA480150.025.7423428
TATTCAC502700.024.6885419
CCGGGAA433050.024.6102561
ATATCGC198200.024.3230824
CGGGAAC456900.024.2618081
Produced by FastQC (version 0.11.5)