FastQCFastQC Report
Thu 28 Jul 2022
HHFK3DRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFK3DRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796007982
Sequences flagged as poor quality0
Sequence length24
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCTCGGTGGTCGCCGTATCAT65092910.3624310729817235Illumina Single End PCR Primer 1 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGG64008150.35639123345499696No Hit
CGATCTCGGTGGTCGCCGTATCAT46814830.26066047851228313Illumina Single End PCR Primer 1 (95% over 24bp)
GGGGGTGGGTGGTCGCCGTATCAT45538740.25355533191611396No Hit
CGGTCTCGGTGGTCGCCGTATCAT21889820.1218804160080843Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)