FastQCFastQC Report
Thu 28 Jul 2022
HHFK3DRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFK3DRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796007982
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACACTC1313710557.314614206430627No Hit
TCGCAATT1195985696.659133489307621No Hit
CATGGCAG1186104376.604115248302945No Hit
CTGCAGCC1159896306.458191230911801No Hit
CTTTCGAC1145626906.378740581788795No Hit
AAAGCATA1145349096.377193762382734No Hit
CAGTCTGG1125555756.26698634572104No Hit
TGTAGCGG1085769606.045460882589775No Hit
AGAACGCC1048645025.838754785667763No Hit
ACGGGACT1045611225.8218628785581865No Hit
ATTGGGAA1006067095.601684959549361No Hit
TGCATCTG944863895.260911418376981No Hit
GAACATGA928061455.167357045743909No Hit
GTCTTTGA880733384.9038388961903845No Hit
GGGGGGGG701711743.9070635934400877No Hit
GCCTTTAT647964583.607804567095738No Hit
GGCATACT375655232.0916122520885323No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)