FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n02_vs_1602_05.3520000004add3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n02_vs_1602_05.3520000004add3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29558619
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG97850.020.673859145
AGAGCGG124700.016.280575145
GCGTCGG150850.014.756088145
TCTCGGG103150.011.387384145
CGGTGGG80750.010.056658145
GATCGGG104500.08.395514145
CCTCTTA231650.07.79246236
AGCTTCC362750.06.57618471
TCGGTGG358750.06.3260207145
GCTTCCT336450.05.925832
GGTCGCC274150.05.7391777145
GGCCCCC81950.05.6625123145
CTCGGTG347950.05.584652145
GTGTAGG780300.05.5752945145
AGATCTC432300.05.568408145
CGTGTAG789150.05.5678973145
TAGATCC183050.05.545446145
GTCGTGG159100.05.5143375145
AGCGTCC99600.05.4598427145
AAAGAGG432150.05.4193373145
Produced by FastQC (version 0.11.2)