FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n02_vs_1602_04.3520000004adb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n02_vs_1602_04.3520000004adb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31104618
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGC149850.019.157947
CTACGCA169500.017.065598
TACGCAG164800.016.4084789
AGCTACG186200.015.5347276
GCGTCGG73450.014.116848145
AGAGCGG91150.011.614209145
GTAGGGG88350.011.161585145
CGGTGGG57100.09.270011145
TAGGTAG625850.08.3186171
GTAAGCT326500.07.59372233
TGTAAGC288250.07.52018172
TAGGGAC103850.06.98090278
AGGTAGT391050.06.87809372
AAGCTAC305000.06.70286375
CTATGAC192450.06.66754157
TAGTAGG283650.06.44063765
AGCCGGG45500.06.40537880-84
CACCCCC68550.06.3465433145
TAAGCTA344150.06.2352774
CGCAGTT223750.06.09134058
Produced by FastQC (version 0.11.2)