FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n02_vs_1602_03.3520000004adfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n02_vs_1602_03.3520000004adfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14387925
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG52650.041.038555145
GCGTCGG55950.039.654774145
TCTCGGG50700.030.175098145
GTAGGGG93500.025.590366145
CGGTGGG68400.024.698694145
AGAGCGG101500.018.930147145
GAACACC133900.016.1343335
TGGGTGT1111750.015.881561
TGAACAC152250.014.09464
GAGGTCG66700.013.8039148
TAGATCC111350.013.674241145
CGTATCC67950.013.444828145
GGGTGTG1349300.013.2339912
AGTGAAC179100.012.3868472
GTCGCCC27850.012.236208145
GTGTGGT592750.011.8391944
GGGTATG68950.011.77656652
GATCGGG47250.011.508896145
TAGGGTG54900.011.4907471
GGCTCCC122850.011.365911520-24
Produced by FastQC (version 0.11.2)