FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n02_vs_1602_01hp.3520000004ae18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n02_vs_1602_01hp.3520000004ae18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837939
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG163730.8908347883145197Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCTCCTTTCGGGGTTCCGGCTCCCGTGGCCGGGCCCCGGAACTTTATA26470.14402001372189174No Hit
TAAACTCCTTTCGGGGTTCCGGCTCCCGTGGCCGGGCCCCGGAACTATTA25170.1369468736448816No Hit
TTATTCTCCTTTCGGGGTTCCGGCTCCCGTAGCCGGGCCCCGGAACTTTA24110.13117954404362714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG37200.030.986017
TTTCGGG37250.030.7506358
TAAACTC20550.029.990081
CCTTTCG39700.028.6695336
TTCGGGG44050.026.9911389
GAGCGTC107850.025.8799749
AGAGCGT109450.025.5016488
CTTCTCC21800.024.6119351
AAGAGCG113650.024.5585527
CATCTCC17800.024.4400331
GATCGGA116050.024.2413651
TCGGAAG118600.023.7175753
TCTCCTT43500.023.49923
AAACTCC25800.023.0418532
CGGAAGA122150.022.9683064
ATCGGAA124550.022.7009562
TCCTTTC51450.022.6856885
GAAGAGC126550.022.2270096
GGACTCC7300.021.8485743
AACTCCT27750.021.6839473
Produced by FastQC (version 0.11.2)