FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n02_vs1508_5a.3520000004ad90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n02_vs1508_5a.3520000004ad90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83428364
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2454430.2941961081725155Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG265200.036.390556145
GTAGGGG325800.028.99863145
TCGCCGG219350.027.998186145
TCTCGGG248500.023.721836145
CGGTGGG277000.020.338802145
GATCGGG207750.017.241302145
AGAGCGG561500.016.838833145
TCGGAAA798600.010.831667145
CGCGTCC90150.09.732038145
GTCGTGG310550.09.526048145
GTCGCCC160000.09.244733145
AGCGTCC201350.09.21876145
TAGATCC564050.08.008563145
CGGTTAG167100.07.6369576145
CGTATCC353550.07.1369486145
GCCCGCC117300.07.0467844145
TCCCGGG87850.07.0155435145
CGCAGGG163200.05.820165145
GAGCGTC7386150.05.6644169
AAAGAGG1797450.05.5587425145
Produced by FastQC (version 0.11.2)