FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n01_vs_1602_05.3510000004add6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n01_vs_1602_05.3510000004add6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29558619
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTTA239700.06.62286576
ACACGTC844400.06.318791145
CAGTCAC491200.06.1838655145
AGCTTCC366450.06.1190191
ACGTCTG793800.05.853981145
ACTCCAG615200.05.8094497145
TCCAGTC562350.05.7624235145
CTCCAGT606400.05.750297145
CGTCTGA791500.05.7427645145
GAACTCC646100.05.677474145
GTCACCT447750.05.515089140-144
GCACACG892650.05.489969145
GCTTCCT343450.05.4664592
AGTCACC475800.05.4093785140-144
AGCACAC987550.05.3808303145
CACACGT866200.05.3501015140-144
CACGTCT825200.05.3136873140-144
CCAGTCA555650.05.179569145
CCTTGTA499200.05.15535145
TAATCTC463800.05.1424437145
Produced by FastQC (version 0.11.2)