FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n01_vs_1602_04.3510000004adbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n01_vs_1602_04.3510000004adbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31104618
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGC159300.019.47647
CTACGCA171750.018.106788
AGCTACG195400.015.9152476
TACGCAG156600.010.8781939
CTATGAC216250.09.8218257
TAGGGAC96950.07.925728
TGTAAGC293500.07.8788572
GTAAGCT329950.07.49181273
TACGCAA219650.07.3926059
AAGCTAC310050.06.897175
TGCGGTG140400.06.71235945-49
TAGGTAG507650.06.6616221
TCTATGA335500.06.54682546
ACGCAGT199250.06.43955147
TAAGCTA350750.06.3241834
ATCTATG363950.06.25415475
TATGACA360900.06.24675048
AGGTAGG401100.05.87369442
CACGTCT492100.05.8485265145
GCGTGTG127300.05.809544105-109
Produced by FastQC (version 0.11.2)