FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n01_vs_1602_03.3510000004adf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n01_vs_1602_03.3510000004adf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14387925
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACC125100.016.8622345
GAGGTCG61600.014.7098688
TGAACAC146100.014.4881214
TCCGGGC44650.014.1246365
GGGTATG61550.013.8973952
TAGGGTG51550.013.3732341
TTCGGGG131200.012.3211299
AGTGAAC180500.012.2490412
CTTTCGG140900.011.7815917
TTTCGGG136750.011.5030198
GGCTCCC126950.011.36326420-24
TCGGGGT112650.011.33847810-14
GCCGGGC96200.011.3181230-34
GCTCCCG108850.011.28152320-24
TAGTGAA191350.011.073781
GGGGGCC36100.010.76527655-59
CCGTGGC134550.010.76443625-29
AACACCT205150.010.6359046
TATACAC94750.010.5691821
AGGTCGA95550.010.4695469
Produced by FastQC (version 0.11.2)