FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n01_vs_1602_01hp.3510000004ae1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n01_vs_1602_01hp.3510000004ae1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837939
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC171850.9350147094109217TruSeq Adapter, Index 5 (100% over 50bp)
CTTCTCCTTTCGGGGTTCCGGCTCCCGTAGCCGGGCCCCGGAACTTTAAA24020.13068986511521874No Hit
TAAACTCCTTTCGGGGTTCCGGCTCCCGTGGCCGGGCCCCGGAACTATAA20300.11044980274100501No Hit
TTATTCTCCTTTCGGGGTTCCGGCTCCCGTGGCCGGGCCCCGGAACTTTA18650.1014723557201844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG37100.031.0695727
CCTTTCG37450.030.9727866
TTTCGGG37500.030.7381678
TAAACTC20750.027.9706861
TTCGGGG43500.026.4984199
CTTCTCC21850.025.8984871
GAGCACA110750.025.3325759
AACTCCT24600.025.0493183
AGAGCAC115100.024.5011448
CATCTCC17000.024.3252411
TCGGAAG122000.023.5313843
CGGAAGA122700.023.3380534
GATCGGA122950.023.3077181
GGACTCC5750.022.6942983
TCTCCTT47950.022.5273323
CGGACTC5800.022.4986572
TCCTTTC51900.022.4890255
GAAGAGC127250.022.4465966
ATCGGAA128450.022.3497772
AAACTCC26650.022.0343342
Produced by FastQC (version 0.11.2)