FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l02n01_vs1508_5a.3510000004ad93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l02n01_vs1508_5a.3510000004ad93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83428364
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2571020.3081709716853611TruSeq Adapter, Index 7 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAG155450.08.067885145
GAGCACA7465900.05.87807949
AGGGACC206900.05.71087039
AGAGCAC7688000.05.7054388
GAAGAGC8580600.05.16515066
CCAGGGA259100.05.14784675
AAGAGCA8707850.05.13628867
CGGAAGA8764350.05.0634774
TCGGAAG8734250.05.06266743
TCCGGGG196250.05.06041538
CGGGGTT179800.05.004041
Produced by FastQC (version 0.11.2)