FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n02_vs_1602_06.3520000004ae25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n02_vs_1602_06.3520000004ae25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115144
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5378746.71281178350587Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCCG1810.15719446953380117Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCCGGTGGTCGCCG1740.15111512540818453Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1510.1311401375668728Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1430.12419231570902522Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1380.1198499270478705Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG1380.1198499270478705Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAATCTCGGTGGTCGCCG1230.1068227610644063Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT62200.0139.441078
GAGCGTC61950.0139.184379
TCGGAAG63250.0139.129553
GATCGGA63900.0138.909041
ATCGGAA64400.0138.333472
CGGAAGA64100.0137.171544
AAGAGCG63750.0136.960787
GAAGAGC63950.0136.645846
GGAAGAG64200.0136.452625
TCGGAAA1200.078.527793
CGGAAAA1350.064.433054
CGGGAGA451.19043296E-464.433054
TAGCGTC502.0001922E-458.014959
GAGCGTT502.0001922E-458.014959
AAGCGTC1153.8198777E-1156.753759
GAACGTC953.1004674E-853.4348229
AAAGCGT1301.2914825E-1050.205258
GATCGAA450.00960784848.345791
CCACGGG450.00960784848.34579145
TATCGGA450.00960784848.345791
Produced by FastQC (version 0.11.2)