FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n02_vs_1602_03.3520000004ae0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n02_vs_1602_03.3520000004ae0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14386509
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG50100.041.827534145
GCGTCGG52800.039.413963145
TCTCGGG50050.032.452343145
GTAGGGG88150.024.677494145
CGGTGGG63550.020.766245145
AGAGCGG94500.017.648108145
TGGGTGT911000.015.2314991
GAACACC134700.014.6385925
GAGGTCG65600.013.9240218
TGAACAC152300.013.4229264
GGGTATG66400.013.1012022
GGGTGTG1084800.012.9643292
GTCGCCC26350.012.383232145
AGTGAAC179500.011.9139432
TATACAC97550.011.4449151
GTGTGGT495100.011.0987554
TCCGGGC47000.010.95111755
TAGATCC104900.010.921536145
GCCGGGC91750.010.88863530-34
TTCGGGG127800.010.7775679
Produced by FastQC (version 0.11.2)