FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n02_vs_1602_02.3520000004ade0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n02_vs_1602_02.3520000004ade0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35797120
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1128010.3151119419662811Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG120050.039.68184145
TCGCCGG86050.029.407843145
TCTCGGG115050.027.352167145
GTAGGGG289200.017.324804145
AGTTCCG164700.016.7700772
GGGCCCG185250.016.201179
CGGTGGG171250.015.877763145
GGGGCCC170350.015.8734378
TATACAC215450.015.2437491
TGGGTGT4019150.014.9631231
AGAGCGG307100.014.449747145
TAGGGTG192500.013.9727941
GGGTGTG4926700.012.4514692
TCCGGGG243200.012.4301615
CCGGGGC220650.012.3534486
CCGGCTC170800.012.30876358
TATACCC218200.012.0944661
CGGCTCC179600.011.5845679
GGGTTCC183150.011.2412623
CGGGGTT174200.011.1955181
Produced by FastQC (version 0.11.2)