FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n02_vs5b.3520000004adad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n02_vs5b.3520000004adad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71394175
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1131090.15842889143266942Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG132800.016.871552145
TCGCCGG142150.015.914842145
GTAGGGG207600.012.783458145
TCTCGGG178850.012.000446145
CGGTGGG194400.010.667543145
GAGCGTC2800100.06.759839
AGAGCGT2897100.06.5910528
AAGAGCG3071400.06.23825657
TCGGAAG3233400.05.8898183
AGAGCGG360450.05.833747145
GATCGGA3245250.05.792751
CGGAAGA3482700.05.5244124
GAAGAGC3579250.05.49085476
ATCGGAA3558850.05.47953652
CGCGTCC73850.05.2037926145
Produced by FastQC (version 0.11.2)