FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n02_vs1602_01.3520000004adc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n02_vs1602_01.3520000004adc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25888581
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG58300.022.38686145
TGGGTGT2696050.017.3503671
GGGTGTG3175600.014.73364452
TGTGCGG79650.014.3806445
TCTCGGG54900.014.263977145
GTGTGGT1410000.013.4706714
GGTGTGC102450.013.1615793
GTGTGCG95350.012.6210194
TCGCCGG51350.012.4260025145
TGTGGTG1538000.012.3595
GTGGTGT1495600.012.3022126
TGGTGTG1520500.011.5238417
TAGGGTG111000.011.4956081
TATCATA688350.011.2267729
CTGTTAG148000.011.072252145
GTAGGGG164900.010.948836145
GGTGTGG3480050.010.9011493
TATACCC156700.010.5489231
TGTTGGA440500.09.8250665
TATTATC843050.09.802996
Produced by FastQC (version 0.11.2)