FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n01_vs_1602_06.3510000004ae28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n01_vs_1602_06.3510000004ae28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115144
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5435247.20350170221636TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC7650.6643854651566734TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC5440.4724518863336344TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC3470.3013617730841381TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC2880.2501215868825123TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC1980.17195859098172722TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1960.17022163551726532TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC1950.16935315778503438TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1920.16674772458834156TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGC1770.15372055860487738TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGC1740.15111512540818453TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC1730.1502466476759536TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGC1710.14850969221149168TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGC1600.1389564371569513TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1530.13287709303133466TruSeq Adapter, Index 6 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1350.11724449385117765TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1180.10248037240325157TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65250.0138.911471
GAGCACA65650.0137.935539
TCGGAAG66050.0136.990523
ATCGGAA66150.0136.783432
AGAGCAC66150.0136.783438
CGGAAGA66200.0136.680114
GGAAGAG66600.0136.076775
GAAGAGC66750.0135.87956
AAGAGCA67200.0134.969597
TATGGCC350.003563309362.121628145
CCATCGT400.006014364354.4273831
AGGTTCT450.00964718348.295834
GACCGCA200.00604383629.027939135-139
TGCCGTC64400.028.20231645-49
TGCTTGA63350.028.17617455-59
ATGCCGT64800.028.07296845-49
CTGCTTG63850.028.0690855-59
TCGTATG64900.028.04960640-44
TCTTCTG64450.028.00781850-54
GCCGTCT64150.027.99588845-49
Produced by FastQC (version 0.11.2)