FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n01_vs_1602_03.3510000004ae0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n01_vs_1602_03.3510000004ae0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14386509
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACC125800.015.5584845
GAGGTCG66000.014.9375428
TCCGGGC43350.014.38114555
TTCGGGG130500.013.9427159
TTTCGGG132400.013.5236258
CTTTCGG135450.013.2726257
TGAACAC145300.013.2708934
GGGTATG64000.012.6859262
CCTTTCG152500.011.6936316
TATCATA304700.011.4910269
TAGGGTG51900.011.4660921
AGTGAAC182400.011.4459492
GGGATCC35000.011.1843279
GCCGGGC90200.011.17100930-34
GGCTCCC122750.011.09068120-24
TCGGGGT108600.011.00047810-14
AGGTCGA98250.010.9935479
GCTCCCG104050.010.979897520-24
GTAGAGG98550.010.4451785
CCGTGGC131400.010.40472225-29
Produced by FastQC (version 0.11.2)