FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n01_vs_1602_02.3510000004ade3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n01_vs_1602_02.3510000004ade3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35797120
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1190550.3325826211717591TruSeq Adapter, Index 2 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG166800.017.0360242
GGGGCCC167750.016.3777758
GGGCCCG191550.015.7809139
TATACAC214900.015.0589761
TAGGGTG175500.013.5197181
TCCGGGG245050.013.1638685
CCGGGGC225350.012.1915776
CGGGAGC120850.011.30092320-24
TTCCGGG294900.011.1352934
CGGGGCC255750.010.9691657
TATACCC216400.010.8974061
CCGAGCG105100.010.0699757
CCGGCTC177700.09.953618
CGGCTCC182450.09.8136679
CCCGAGC108900.09.7851556
CGGGGTT165950.09.225761
GGGTTCC179650.09.1596083
TTAGCCC110200.08.74879559
ATACACC183400.08.7351672
GTTCCGG347950.08.6875526
Produced by FastQC (version 0.11.2)