FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n01_vs5b.3510000004ada0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n01_vs5b.3510000004ada0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71394175
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1150160.16109997769425868TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2875150.06.915929
AGAGCAC2939850.06.7637148
TCGGAAG3194500.06.1723513
GATCGGA3201900.06.0815911
CGGAAGA3352350.05.9401024
GAAGAGC3440150.05.8622516
ATCGGAA3547750.05.6721942
AAGAGCA3669450.05.58877477
GGAAGAG3845250.05.31252535
Produced by FastQC (version 0.11.2)