FastQCFastQC Report
Thu 25 Feb 2016
HHFCMBCXX_l01n01_vs1602_01.3510000004adc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHFCMBCXX_l01n01_vs1602_01.3510000004adc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25888581
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATA686400.012.757689
CTGTTAG149950.012.472985145
TGTGCGG104050.011.9134095
TATACAC181000.011.7866991
TAGGGTG103850.011.599121
TGGGTGT3889150.010.898231
TATACCC145150.010.6984341
TTATCAT845350.010.5904028
TATTATC848250.010.4687366
GGGATCC60300.09.9779859
CAATATA550400.09.7956631
TATATTA919400.09.6428194
GTGTGCG126100.09.6002694
GGTGTGC132250.09.318273
TGTTGGA451000.09.258235
GGGACTT107450.09.2426422
GGGTGTG4865550.08.88413052
CGTACAA132700.08.849652
TGGGACT118300.08.7715191
TACCCAC184200.08.7366593
Produced by FastQC (version 0.11.2)