FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n02_grads_set02_rna_digest_nextseq_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n02_grads_set02_rna_digest_nextseq_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32107027
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCTGGAATTGAATGTAATAGAATGGAGTGGAGTGGACTCGAATA470760.14662210861192473No Hit
TTGAGTCCATTCCAATTCATTCGATTCCAATACATTGCATAACATTTGAA462950.14418961930047278No Hit
TTGAGTCCGTTAGAAGATTCTATTCAATTACATTCCATGACGATTCCGTT435100.13551550568665233No Hit
TTGAGTGGAACGGAATGGACTCGAATGGAGTGGAGTCAAATGGCCTCGAA383340.11939442415518572No Hit
TTGAGTTCTGGAATGGAATCATCGAGTGGACTCGAATGGAATCATCATCA340040.10590827982920997No Hit
TTGAGTTAGCATCGATGGACTGGAAAGCAATGGACTGGAATGGAACTTTC333570.10389314463777664No Hit
TTGAGTCCGTTCCATAACACTCCATTCCTTTTGATTCCATTTCTTGCCTG328080.10218323857889428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA7207900.0139.662051
TTGAGTT7946500.0138.375981
TTGAGTC4114500.0131.98081
TTGAGTG2722050.0127.416161
TGAGTAT4111600.0112.3701252
TGAGTTC3024700.0112.0088352
TGAGTAC1987700.0111.961522
GAGTACT1054500.0110.871033
GAGTATC1446000.0110.7503053
TGAGTTA3166950.0110.116792
ACTAACG444000.0107.449997
GAGTTAC1048750.0107.107733
TGAGTCC2857350.0104.177382
GAGTTCC702550.0102.6995243
TGAGTAG2319150.0102.327422
TGAGTCT1426000.0102.322082
TGAGTTT2309450.0101.830312
GAGTAGC936900.0101.445093
GAGTTCT1351350.0101.4381943
GTGACCG209100.0101.292555
Produced by FastQC (version 0.11.2)