FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n02_grads_set02_dna_digest_nextseq_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n02_grads_set02_dna_digest_nextseq_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38799697
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGA5894741.5192747510373599No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTACATTCC2373510.6117341586456203No Hit
TTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT2312380.5959788809690962No Hit
TTGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTT2167630.5586718885974806No Hit
TGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGAA1396790.36000023402244613No Hit
TTGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGAC1125580.2901002036175695No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCATTCC894430.23052499611015007No Hit
TCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA850100.21909964915447663No Hit
TTGAGTGACTGCCCATGTTATGTTTTCGTTGATGCTTATAATTCTGCAAT811940.20926452080282995No Hit
TTGAGTGCCTGACGGAATGCGTATTGATACGTCTCACATGCACGGGATGG744240.19181593093368746No Hit
TGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTTT732890.1888906503573984No Hit
TGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCATT709590.1828854488219328No Hit
TTGAGCTTCACTTTCTATAATGTGTTATGAAAGCAGTAGGTATTTTTCAT697600.17979521850389707No Hit
TTGAGTTCTGTAATATTTTGGATTTTCTACAACTTCCCCATTACTAGTAA622130.16034403567636107No Hit
TTGAGTATCATGTGCTTGAAATTGGCTCTTTTGCAGTTTGGTGTCATATT605330.15601410495551035No Hit
CAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCAG593070.15285428646517524No Hit
TTGAGTGGAATCATCAAATGGATTCGAATGGAATCATCAAATGGACTCGA588270.151617163402075No Hit
TTGAGTCCCTTCCATTCCAGTCCATTATATTCGAGTCCATTCCATTCAAT561300.14466607819128072No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTACTTTCC551280.14208358379705904No Hit
TGGAGTATTGAATCCGCCATGCAGAATCAGTTTAGCAATTATACAGCATA507200.13072267033425544No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTGCATTCC488470.12589531304844984No Hit
TTGAGTCCGTTTTATTCCAGTCCACTGCATTCTGGTCCATTCCATTTGAT483490.12461179787048336No Hit
TTGAGTCCGTTATTCGAGGATGATTCTATTCGAGTCCATTCAATGATTCC482850.12444684812873669No Hit
TTGAGTGGTTTCGTGATCAGAATTTGCAGAAATCTGTTGCTGTTTCTCGT469140.12091331537975669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG442340.11400604494411386No Hit
TTGAGTTACTAAACATCGCTTTTAACTTATTTAAAGTTGTCTCCAATTGG422050.10877662266280069No Hit
TTGAGTTCTGGAATTGGATGGAATGGAATCAACTCGATTGCAATCGAATG419380.10808847295895119No Hit
TTGAGTCTTCCGAAACTCTTTATAGTGTTCTCGATAGTATGAAGTTTAGT402690.10378689297496319No Hit
TTGAGTATCGAGTCCATTTTAGGATTTGATTCGAGTCCTTTCGATGATTC396960.10231007731838732No Hit
TGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGACT396860.10228430392123938No Hit
TGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTACATTCCA395270.10187450690658745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTT8352050.0138.498551
TTGAGTA7415050.0137.787491
TTGAGTC4127850.0130.511121
TTGAGTG3758550.0115.0722351
AGTATCG1235750.0111.3205264
CCGCCTT338800.0110.476912
GCCGCCT330900.0109.720441
GAGTATC2943450.0109.477933
TGAGTAT4641150.0107.8621142
CGCCTTA350100.0106.9940643
GTATCGA1147200.0104.942455
TATCGAG1150200.0103.68296
TGAGTTA3746850.0102.8612442
GAGTTAC1538100.0102.42853
TGAGTAC2299850.0102.406862
TGAGTTC3646300.0102.13742
GAGTACT1438900.0101.6812443
TAGTTCG477750.099.996826
AGTTACG688250.099.67964
GCCTTAC382550.098.311814
Produced by FastQC (version 0.11.2)