FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n02_grads_set01_rna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n02_grads_set01_rna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79147978
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTACTACGAGGCATGCTCCTAAATCCTAGAACAAATCATTCTGGTA2703700.3416006407643162No Hit
TTGAGTATACGATGGGGCGGCCTCAGGAGAGTTACCACTTCTGAACCTCA2550970.32230387490126405No Hit
TTGAGTCTAAACCAGCACAACCTCCACAACAGGAATCTGGCAACAGCTAC1968270.24868228471989517No Hit
TTGAGTACTGAATCCCTCCATCAAAATCTTCTTCGTCGGCACTATCTTTT1790410.22621045353805502No Hit
TTGAGTCTGTATGTCTGATGAAGTTATCGAAGATATCGATGACATTAATA1747910.22084076487715198No Hit
TTGAGTAGAACCATTCGCAGGCGTCGGAGATGTAAAGTTAATTGTAGGTA1734310.21912246450566303No Hit
TTGAGTCCAGTACACTGCCTTAGGAACTGAGGGAGTTTTGTTTTGTTGGA1619480.20461419747198092No Hit
TCAAGTAGTGAGAATCAACTGTATCTACACTGCCCTCTCATTTATTTTTA1512460.1910926896957494No Hit
TTGAGTTTAAAACAAGTTAATAAATTATGTTTGAATGATTACTTGGATTA1492300.18854556208624812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1364630.1724150173488955No Hit
TTGAGTTCAAATCATCCATTCTCCACCAAGCAACTAAAGAATTATCAAAG1337710.16901379337827177No Hit
TTGAGTATGCCAATTCGCTCTCATTTCGCAGAAAATAAAACACCTATTGT1292430.16329286390613795No Hit
TTGAGTGCTATATAGTTCAGGTGGTTCTGCTACATTGAGCGAATTATTTG1249020.15780820073508384No Hit
TTGAGTACTGAATCAATAGTGAAAAACCTACTGCTAACTTGGCTATATTC1227980.15514988898389798No Hit
TTGAGTCTATACGGGCTCTTTTTTGGTTCCATATGAAATTTAAAGTAGTT1207440.15255475004048746No Hit
TTGAGTATTGAATTGATGAGGGATGCAGAAAGAGATTGTTTAAGATAGAT1181120.14922933343919412No Hit
TTGAGTACTGAATCCTGTGCTCCCTTTAAACCCAATAAATCCGGATAACG1094460.13828022239557403No Hit
TTGAGTACTACGACCATAATCCTTGCTTCCTCTCTTTGGTACTTGCTACA1073960.13569013727678553No Hit
TTGAGTGATGCACCGAAGCTTAGGGTGTGATCTTCGGATCACGCGGTAGC1053760.13313795584266222No Hit
TTGAGCAATCCATGTTTGTGAATCTGCCTACTTGCTAAAATTTACTTGTA1021070.12900771767031116No Hit
TTGAGTCCAGTACTGTACCATAGCTCCTAGGAGAAATATAGAGTTTAGTT980850.12392609701286368No Hit
TTGAGTTGTGTCTGTTGATACCATAAAGTATTCCTTTGTAGAAGTTAAGT962890.12165692975757383No Hit
TTGAGTCCATCCCACTATATATGTTAGTCACATTTTCAGTTCTTTCCCTC933080.1178905669580087No Hit
TTGAGCAAGATTAGTAAAATTATTCGAATAATATCTTGAAGAACTATTTG930060.11750900319904571No Hit
TTGAGTAAGCATTTTCGCATGATTAAAGGTTTACCGGTGTAAGATGGGGA920090.11624933741200565No Hit
TTGAGTCACTAGCCCTCCTTTAAAGATGCACTGAAATGTCTACCCTAAGC847280.10705011314376219No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA14647400.0139.61551
TTGAGTT19715800.0137.91061
TTGAGTC8392600.0134.172131
TTGAGTG7826500.0132.88831
TGAGTTA8891500.0115.388442
TGAGTAC3787700.0113.437782
TGAGTAT5945550.0112.4962162
GAGTACT2363700.0112.446063
TGAGTGC3164150.0111.751272
TGAGTTC5853350.0111.220962
TGAGTAG5999500.0110.9802252
TGAGTCC4922700.0110.108062
GTTTGCG846050.0109.383535
GAGTGCC1261350.0107.245063
GAGTAGC3900350.0106.1245963
TGAGTCG767000.0104.780222
TGAGTCT2404400.0104.662422
TGACGGC641200.0102.6253057
GTTAACG1213250.0102.502485
TGAGTGA4644750.0102.226952
Produced by FastQC (version 0.11.2)