FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n02_grads_set01_dna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n02_grads_set01_dna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74279356
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTACTAACGGACTGGAATCGAATGGCAAGAAATCGAATGTAATGGA9211731.2401467239430564No Hit
TTGAGTCCGTACCAGTCGAGTCCATTCTATTCCAGTCCATTAGTTGCGAT5775470.7775336662854212No Hit
TTGAGTTCAACTACAGTAAGTACTAGTTTATGTAGGTATTCCACTGAAAG4120300.5547032475618124No Hit
TTGAGGCACTTTCACATATTTTGTTGGCTATTTTAAGTTTTCTATGAATT3707830.49917368696626824No Hit
TTGAGTAACAACACCAACTTTTTCGATATCTGGACGTGATTTTAATTCTG3213360.43260471994399086No Hit
TTGAGTAGACACTTTTTCCTGTCTTGACTGCTGCCGTGAAACCCGATCCT3095840.4167833657577753No Hit
TTGAGTTATCCGCAGCGCTAAACCCTGTCATCTCCATGACCCTACTTGAC2949280.3970524461736044No Hit
TTGAGTTCGGAAGAGACCCCTCCAAAGTCGCCACTAGTACACTGCCCGGC2901510.39062131879549417No Hit
TTGAGTTCGGAAGATTGGGCGACCAAAGGGGTGGTAAATGTTACTGTCAT2587700.34837404890801693No Hit
TTGAGTGCTTTGTAAACCGGCTCAACCTTCATCACCATCCCAACTCCCCC2347260.316004355234313No Hit
TTGAGTATCATAATGGATCTCCCAGAAAATGTCTTGTGTCAGTGGGCAAA2056350.2768400415318625No Hit
TTGAGTAGACACTTTGATACTGTTAAGGTTCCGGCGGAAGTAATTGTGCC2041490.27483948568428623No Hit
TTGAGTATCATGTTTTATGGATGAATATAGATGTGATTGAATGTGATTGA2040030.2746429303991273No Hit
TTGAGTTATCCGCTATCTCCCTGCACTCATCTTGCCCCCAGCTTGGATTC1983610.2670472802699043No Hit
TTGAGTAGACACTGGGGACGCTGCGAAGGACAAGGAAATGGAATTCCAGT1934480.2604330602973995No Hit
TTGAGTATCATGTTTCTTGGCCATTTGCATATCTTCTTTTGAGAATTGTC1901660.25601460518855335No Hit
TTGAGGTAAAGTGATAAAAGTCGACCTGGAGAAGTCTTATAACTCAGTTG1897010.255388590068013No Hit
TTGAGTAGACACTGATAGCATTCACCTGAGCTTCGTGAAGTTCGATTTCC1896740.2553522408029493No Hit
TTGAGTATCATGTTGAATAAGCTTCTGATATCAAAATATTTATCAAAAAA1896140.2552714646583635No Hit
TTGAGTATCGGAACTTAATTAGAATCTGAAGGATTTGATGCAGCAGAAAT1875860.25254123097136166No Hit
TTGAGTAGACACTCCTTCGCACTGACTAAATACCCTGTAGATTCTCCAGA1832760.2467388112519446No Hit
TTGAGTTGACCCGATCAAGTGCTCCTTACCACGTAAAACACATCAAAGAA1820420.24507751521162893No Hit
TTGAGTATCGAGCGTTATCCATTTTGAAATTCTTTTCCGCCGTATACTTT1811290.2438483715448475No Hit
TTGAGTTCATCTGTGGTTAACTGAGAGAGAATTTCTCACCCAAAATAATT1771200.23845117881743616No Hit
TTGAGTACTAACGAATCGAATGGAATGGAATGGATTCTAATTGAAAGGAA1689780.227489855997136No Hit
TTGAGTAAAACAACATCCCATTTTTCCAAATCACTAATATCGATATGTGC1682670.22653265868379366No Hit
TTGAGCAGCACAAGCAAGCAGCTCAGCTGGAGTACATGTCCCACCAGTCG1677370.2258191360732853No Hit
TTGAGTTCATGACCAACAACCCAAAAGCAAATGCAACAAAAACAAAGATA1671920.22508541942663046No Hit
TTGAGTATTTTGCAATTCCGATAATTTGTAAAAGCCAAAAATTAAGAGAC1625480.21883334583568548No Hit
TTGAGTAATCAACTACTTCATATTCATTTTGATTTAATAGATTCTCAATT1624480.21869871892804238No Hit
TTGAGGAATCCTCCTGCCACGGCCTCCCAAAGTACTGGGATTGTAGGGGG1601660.21562653289562717No Hit
TTGAGTTATCCGCTCATGCATACATAAGAAGACTTCAAGGAGCTCTTCTG1568650.2111824986743288No Hit
TTGAGTTCGGAAGCTGCGATCGGGCTACTCTAGGAAAGAACACGAATGAA1566570.2109024747064312No Hit
TTGAGTAGACACTTTTGGGAACTTTCGCCGTATTAGTTTCAGGTGCAGTA1555400.20939869214805792No Hit
TTGAGTGAATAAGATATGATTAAGTTGCATATAGATGCTTTGGCGAAATT1553440.2091348234090775No Hit
TTGAGTTCCAATGACTTGAAGATAAAAAAGAAGAAAAAGTTAAAGAAGTA1530120.20599532392284067No Hit
TCAAGTGCGATATGGGGCTTTGCAGTTGGTGCGGGTGGCGAAGGCGTTGA1478340.19902434264508162No Hit
TTGAGTAAGCACCCTCCCTATCAGGATCCCTTTGTTGCTCAATCCCTACA1471780.19814119013094297No Hit
TTGAGTTGTGCGGCGGGGATACTTCACCACCAATCAGCTTCATCAACACC1452070.19548769378129774No Hit
TCAAGCTGATATGTACACCAAAACTGCCGAACAAACACGTGCTACGATAA1411880.19007703836312206No Hit
TTGAGCATCATCAAAAATAATCGCTATACCGCTGCTACGTAGCTTCTCCT1399940.18846959308586358No Hit
TTGAGTCCATTCCATTCCATTTCCTTCAATTCTATTCCATTGGCGTCCAT1358140.18284218834638252No Hit
TTGAGTAGACACTCCTGCTAATGTGATGCGAGCCTAGCGCTCGCTTGCAT1349550.1816857432097284No Hit
TTAAGATCCGGTTCATCTTGCCAAATTTTAGCAAATGGTCTGAATTGTGA1335860.17984270084409457No Hit
TTGAGTTATAGCAAACGGTGTATGGTTTTTTGAGGAGGTCATATTGTTGT1327740.17874953035403268No Hit
TTGAGTTACACGTTCAACCGGTGTCTCACGTCCAAAGAGTGAAACAAGTA1319390.1776253956752129No Hit
TTGAGTATGATGGGGTGGTGGTTGTGGTAAACTTTAATAGTGTAGGAAGC1318020.17744095681174188No Hit
TTGAGTGAATACGAAGAACAAATGAAACCATTCTACTTAATGCTTCTGCC1287810.17337387793184422No Hit
TTGAGTTTCATGCTTTCTTCTAAACTCTTTTTTTCTTCCTGCGCTCTTTT1271550.1711848444135676No Hit
TTGAGAGTAGCTGCATATAGGTCAGCACTAATTTCATCAGGCAATCCTTT1260720.169726835003793No Hit
TTGAGTATGCTTCTTCCATGTTCTTTCCTGCTTTCCTTGCTGTTCCCGTT1234580.16620768763800267No Hit
TTGAGTTCGGAAGTTGGCAGACGGTCTTACATTACGGCTGCGCAATTAGA1198310.1613247696977879No Hit
TTGAGTAACACGCCTCGTGTAACTGGATTCGTTGGTGCTGGTGTAAATCC1176950.15844913895053156No Hit
TTGAGTATCATGTCCCCCGCACCAATAGGATCCTCCCGAATCAACCCTGA1164270.15674206976161723No Hit
TGGAGAAGTCTTATAACTCAGTTGAGATTTGTTGGACAGTAGCTTTGTGA1147310.15445879740799046No Hit
TGAGTACTAACGGACTGGAATCGAATGGCAAGAAATCGAATGTAATGGAA1144770.154116845062577No Hit
TTGAGTCACCACGACAAGAAAGCATCGAATCACCAGGAACACACACCAGA1137380.1531219522150946No Hit
TTGAGTTTGAACTTGATGCTGACGAGGTGGTGAGTTACAAAAGAGACTAT1127420.15178106821496945No Hit
TTGAGTATCGGAACCCTAATGACCCCCGCCGAGCAACTACAAGAGATCAT1080550.14547110505373795No Hit
TTGAGTCCATTCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT1079600.14534320949147703No Hit
TTGAGTATAACGTTTGGGTGTTTGGATGGACCGGAGCCATGCAGAGATAC1059360.14261836088078092No Hit
TTGAGTGTCCAGGCGTCGCCCCCCGGGGCCCAACTCTCTACGGAGATGAG1051610.141575002346547No Hit
TCGAGTGGTGCGATGTTCTGGTCTTACAGATGGTCTTCTCGATTGATCTC1039370.13992716899699562No Hit
TTGAGTTACGCAGAGGGGATGAAACGGTTTTTTGAAGCGCAACGTATTGA1029010.13853243423381323No Hit
TTGAGTTACACGTTTTGCCTAAATTCTCCAAACTGGAGGAGAAAGATATA1015710.13674189636216016No Hit
TTGAGTCTGTGAGCGTATGGACCACATTGGAAGTTCGGCATTGACAAGAC984710.13256846222522448No Hit
TTGAGTTCCAATGTGGAGTGCGATTGGACTGGTAACACAAGCCTACTTTA979420.13185628588379253No Hit
TTGAGTGCTATATGTGAGCATGTGCGTGAGACGTTTTCAGCTGTAAGTGA963720.12974264343379605No Hit
TTGAGCAGCAAGGCCCCTGTAGGGTCAGCTCTTGGCTGTGTGATAGCGCT945890.12734224567051983No Hit
TTGAGTCCAATCGATGATGATTCCAATCGAATCCATTCAAAGATTCCATT936600.12609156169851554No Hit
TTGAGTAGACACTTCGAGCTGCTGGACTACGGTAAGAACGTAGCCAATAA934000.1257415317386435No Hit
TTGAGTGTAATCGAATGGACTCATCCAATGGAATCAAATGGAATCATCAA921340.12403715508788203No Hit
TTGAGTACATGGATCCCATATCGTTGGCCTTCTCCCACGCTGTTACGCAG916970.12344883550148174No Hit
TTGAGCGAGCTGTCCAGTTCGCCCTAGCAACAGGCTGTAAGTTCTTTATT889360.1197317865814561No Hit
TTGAGTTAATCGCGTTCTCTAATAAATTTTTAGTGCTTTCATTTATTTTA868880.11697462751292566No Hit
TTGAGTGAAGACTGGAAGCTAGAAAGGGTTGTGTATGAACAAGGAAGTAG864940.11644419749681191No Hit
TTGAGTAAGACTAACCCCTGTTACAAATGTTAATGTTCCAGATAGTTCAT860900.11590030478993382No Hit
TTGAGTGCAGAACTTGGAGACCAATGTGACCGGCTTACAAAATACCCAAG846850.11400879673754845No Hit
TTGAGTTCCAATGGGGTCTTCGAGTGCAGTTTTGGGATTATTTATAGCTA828940.11159762882166076No Hit
TTGAGTACTAACGCTACCCCATGGCTCCCACAGCACATACACCACCCCAC822020.1106660106207706No Hit
TTGAGTAGACACTGCACCTTCTGCTGGAAGACAGCCTGACCAATAAAGCT816480.10992017755242789No Hit
TTGAGTAGACACTCCGCCCCCATCATGTCGAGGTTCGCAGTCACGGTCGC813820.10956206997809728No Hit
TCAAGCAATTTAACCAGCACCGCCCCAACCTCAGGCACAATCGGCTACTG807140.10866276223504147No Hit
TTGAGGATCAACAGAATCATCACCTTTTTTGCTACCTGCAACAAATGAGT806700.1086035263956785No Hit
TTGAGCCGTCTGTATAAACTGCTGAGGAGACTGATAAGCCGGAAACTCTG802520.10804078592173039No Hit
TTGAGTTATAGCAGCCTCTCAGGAATATTGGGATAATGCCGAAGTGTTAC791920.10661374070071368No Hit
TTGAGTCCTATCCCTGTAAACCATATAATCACTTTACGTAAGCTTGTAAC784940.10567404488536492No Hit
TCCAGACTCTGCGGTAAAGGAAAGCTCATTCTGTAATGGAAAGAAGCTGG772850.10404640557196No Hit
TTGAGTATCATGTGGTAGAATAGGAAATATCTTCCTATAGAAACTAGACA770370.10371253084100515No Hit
TTGAGTTCCAATGGTATTGCAGTATTGTAGCATGTTTAGAGCTCAATCGC768490.10345943225463614No Hit
TGAGTCCGTACCAGTCGAGTCCATTCTATTCCAGTCCATTAGTTGCGATT767210.103287109812853No Hit
TTGAGTATCGAGCTTTACATATTCTATTGTGGTAATGACTTCTTCGTAAA760620.10239991849148504No Hit
TTGAGTTAGAATGGGTTTATGAGTAATCATGAGGTAGCACAACTCTTCAA756230.10180890636693188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA18181600.0139.827561
TTGAGTT20040500.0136.098721
TTGAGTC7742250.0134.410141
TTGAGTG8031700.0126.471341
TGAGTAC5645200.0122.550282
GAGTACT4528850.0120.044033
TGAGTTA11129600.0117.833922
TGAGTAG6832500.0115.726472
TGAGTAT6483650.0115.607012
TGAGTCC6312000.0115.603062
GAGTGCC1631600.0114.794913
AGTTACC2345100.0113.932724
GAGTTAT3266950.0113.8260353
TGAGTTC7566550.0113.451382
GAGTATC4205050.0111.6988453
GAGTTAC5519050.0111.1478963
AGTAGAC4056250.0110.697494
GTAGACA3992950.0110.278935
CGTACCA1272100.0109.9299858
GAGTTCG2192100.0109.861293
Produced by FastQC (version 0.11.2)