FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n01_grads_set02_rna_digest_nextseq_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n01_grads_set02_rna_digest_nextseq_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32107027
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2396430.7463880103255901No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2068740.6443262404831192TruSeq Adapter, Index 19 (97% over 38bp)
TTGAGTTCTGGAATTGAATGTAATAGAATGGAGTGGAGTGGACTCGAATA438160.13646856808012775No Hit
CCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATCGC380030.11836349718707996No Hit
TTGAGTCCGTTAGAAGATTCTATTCAATTACATTCCATGACGATTCCGTT354620.1104493418216517No Hit
TTGAGTGGAACGGAATGGACTCGAATGGAGTGGAGTCAAATGGCCTCGAA342790.10676479015014377No Hit
TTGAGTCCATTCCAATTCATTCGATTCCAATACATTGCATAACATTTGAA333040.10372807173956032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA4823500.0136.415541
TTGAGTT5431750.0134.737411
TTGAGTC2900800.0124.574551
TTGAGTG1911850.0120.311781
GTGAGTA920550.0110.636031
CGCCTTA281550.0102.84743
CCGCCTT282200.0102.8160252
TGAGTTC2657050.099.400442
TGAGTAT3501450.098.868312
GTGAGTC412150.096.982421
TGAGTAC1763550.096.428832
GAGTATC1290600.095.762843
GCCGCCT307550.094.5106351
GAGTACT947700.094.1503753
TGAGTTA2764350.093.9763952
GCCTTAC313150.092.955244
GAGTTCT1211300.090.235033
ACTAACG396100.089.668377
TGAGTTT2026900.089.125952
GAGTTAC965550.089.0083853
Produced by FastQC (version 0.11.2)