FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n01_grads_set02_dna_digest_nextseq_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n01_grads_set02_dna_digest_nextseq_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38799697
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGA4893051.2611052091463497No Hit
TGAGTATCATGTGAATGGAATGCACTCGATTTCAATGGAATGGAATGGAA2988470.7702302417464755No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCC2756030.7103225574158479No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2118140.5459166343489744TruSeq Adapter, Index 3 (97% over 36bp)
TTGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTT1454770.3749436496888107No Hit
TTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT1453400.3745905541478842No Hit
TCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA1207930.3113245961688825No Hit
TGAGTATAACCCATTTGTAACCAAGGACCTCAGAACTTTGTTGGCATTTT1002340.25833706897247166No Hit
TGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCATT869860.22419247243090581No Hit
GCAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCA774240.19954795007806375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG688880.17754777827259835No Hit
CAAGAAGCATAGAGCTGTCTACATGGTATAATTTGAAGCCAGCTTTGCAG682860.1759962197642935No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTGCTTTCC660020.17010957585570835No Hit
TGGAGTATTGAATCCGCCATGCAGAATCAGTTTAGCAATTATACAGCATA576430.14856559317976117No Hit
TTGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCATTCC572600.1475784720689958No Hit
TGAGTATCGAGTGCATTCCATTCCATTCCATTCCATTCCATTTCTTTCCG566460.14599598548411347No Hit
TTGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGAC526670.13574075075895567No Hit
TTGAGTGGAATCATCAAATGGATTCGAATGGAATCATCAAATGGACTCGA483650.12465303530592003No Hit
TTGAGTCCGTTATTCGAGGATGATTCTATTCGAGTCCATTCAATGATTCC470040.12114527595408799No Hit
TCAAGAATTTAGAGACAGCATACAAAAACTTTTTTTCTGACTTGAAAAAG468400.1207225922408621No Hit
TCGAGTGCAATGGAATCGAATGGAATGTAATAGTATGGAATGAAATCCAA456600.11768133137740741No Hit
TGAGTAGCATCAGAAAACCTGCAGGACAGAAGGTTTTCATTTTCTTGACT435820.11232561945006941No Hit
TGAGTGGAATCATCAAATGGATTCGAATGGAATCATCAAATGGACTCGAA427620.11021220088393989No Hit
GTGAGTTACGATTGAGGTAATGGATATGTTAATTAGCTTGATTTAGCCAT414590.10685392723556579No Hit
TTAAGTTTAATGGAAATTCAAGAAGTTAATACGACTATTTAAGAATTATT404530.10426112348248494No Hit
CAAGAACATTCTGGAGAGGTAAGCCAAGGCCAGATCACATAGTCTCGTGG402710.10379204765439277No Hit
TGAGTCCGTTATTCGAGGATGATTCTATTCGAGTCCATTCAATGATTCCA392130.10106522223614271No Hit
CGAGTGCAATGGAATCGAATGGAATGTAATAGTATGGAATGAAATCCAAT389730.10044666070459261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTT5423300.0134.308641
TTGAGTA5055750.0133.575161
TTGAGTC2858500.0121.635821
GCCGCCT455600.0118.384971
CCGCCTT462650.0116.73172
CGCCTTA478900.0112.8464663
GTGAGTA665250.0110.579781
GCCTTAC529900.0102.259384
TTGAGTG2704950.0100.974951
AGTATCG1131000.094.924384
TACACTG605750.089.9438868
TGAGTAT4216600.089.750082
GAGTATC2796450.089.175243
GTATCGA1067200.088.955025
GTGAGTT1100500.087.71711
TATCGAG1085900.086.882356
TGAGTAC2007750.084.285562
TGAGTTA3241750.084.21222
GTGAGTC393400.084.005111
TGAGTTC3193250.083.72712
Produced by FastQC (version 0.11.2)