FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n01_grads_set01_rna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n01_grads_set01_rna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79147978
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT33459794.227497763745777TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4755610.6008504727688685No Hit
TTGAGTACTACGAGGCATGCTCCTAAATCCTAGAACAAATCATTCTGGTA2204050.27847205395442953No Hit
TTGAGTATACGATGGGGCGGCCTCAGGAGAGTTACCACTTCTGAACCTCA1934340.24439537798426134No Hit
TCAAGTAGTGAGAATCAACTGTATCTACACTGCCCTCTCATTTATTTTTA1453820.18368378279985878No Hit
TTGAGTTTAAAACAAGTTAATAAATTATGTTTGAATGATTACTTGGATTA1370580.17316677376142195No Hit
TTGAGTCTGTATGTCTGATGAAGTTATCGAAGATATCGATGACATTAATA1230030.15540889749577683No Hit
TCAAGGTAATCACAAATTTAAGGCATATGCGCAAGACCTTGCTGGAAATG1152020.14555267602666994No Hit
TTGAGTACTGAATCCCTCCATCAAAATCTTCTTCGTCGGCACTATCTTTT1135300.1434401773346629No Hit
TTGAGTCCAGTACACTGCCTTAGGAACTGAGGGAGTTTTGTTTTGTTGGA1116640.14108256814848763No Hit
TCAAGTTGGCAATTCAATATTTGTTAATTTGACTATGAACGATGCAAATA1091780.13794161614589825No Hit
TTGAGTCTAAACCAGCACAACCTCCACAACAGGAATCTGGCAACAGCTAC944330.11931195513295362No Hit
CAAGGTAATCACAAATTTAAGGCATATGCGCAAGACCTTGCTGGAAATGT927170.11714386437010431No Hit
TGAGTATACGATGGGGCGGCCTCAGGAGAGTTACCACTTCTGAACCTCAT926930.11711354142237215No Hit
TGGAGGATACATTCATAGGAGCAGGATGCTTAGAGAAAAGAGCATGTGAG897120.11334717862280701No Hit
TTGAGTGCTATATAGTTCAGGTGGTTCTGCTACATTGAGCGAATTATTTG797070.1007062997869636No Hit
TCAAGAAGATCAAGAGGCGATGAGAGAAATTGCTGACTTTATTGCACAAT792260.10009857737616494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA9039500.0136.699141
TTGAGTT12601400.0134.001171
TTGAGTC5525950.0127.195331
TTGAGTG5028050.0126.703281
GTGAGTA1630700.0107.5311
TGAGTAC3180150.096.6885452
TGAGTTA7247850.096.6290052
GTGAGTC764750.095.8502661
GAGTACT2074350.094.927453
TGAGTAT4885600.094.578092
TGAGTTC4921450.094.471042
TGAGTGC2637700.093.704062
GTTTGCG763100.091.379225
GAGTGCC1138100.090.7903063
TGAGTAG5037600.089.860612
TGAGTCC4214850.089.066762
GAGTATC1857050.087.646963
GAGTAGC3363050.085.391693
TGAGTCT2024000.083.9815442
GTTCGGA515050.083.403275
Produced by FastQC (version 0.11.2)