FastQCFastQC Report
Wed 7 Sep 2016
HHCYTBGXY_n01_grads_set01_dna_digest_nextseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCYTBGXY_n01_grads_set01_dna_digest_nextseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74279356
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTACTAACGGACTGGAATCGAATGGCAAGAAATCGAATGTAATGGA7759581.0446482600091471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5826510.7844050236515243No Hit
TTGAGTCCGTACCAGTCGAGTCCATTCTATTCCAGTCCATTAGTTGCGAT5608810.7550967458576242No Hit
TTGAGTTCAACTACAGTAAGTACTAGTTTATGTAGGTATTCCACTGAAAG3130520.4214522269148375No Hit
TTGAGGCACTTTCACATATTTTGTTGGCTATTTTAAGTTTTCTATGAATT2646350.356269917041284No Hit
TGAGTCCGTACCAGTCGAGTCCATTCTATTCCAGTCCATTAGTTGCGATT2407370.324096778652739No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2326690.3132350797440947TruSeq Adapter, Index 13 (97% over 38bp)
TGAGTACTAACGGACTGGAATCGAATGGCAAGAAATCGAATGTAATGGAA2326630.3132270021296361No Hit
TCAAGTGCGATATGGGGCTTTGCAGTTGGTGCGGGTGGCGAAGGCGTTGA2301970.3099071025871576No Hit
TTAAGATCCGGTTCATCTTGCCAAATTTTAGCAAATGGTCTGAATTGTGA2235880.30100961026102596No Hit
TCAAGCTGATATGTACACCAAAACTGCCGAACAAACACGTGCTACGATAA2178540.29329010337677136No Hit
TTGAGTTCGGAAGATTGGGCGACCAAAGGGGTGGTAAATGTTACTGTCAT2169000.2920057626778563No Hit
TGAGGCACTTTCACATATTTTGTTGGCTATTTTAAGTTTTCTATGAATTG2025430.27267737754753824No Hit
TTGAGTAACAACACCAACTTTTTCGATATCTGGACGTGATTTTAATTCTG1995410.2686358777800928No Hit
CAAGCTGATATGTACACCAAAACTGCCGAACAAACACGTGCTACGATAAC1911140.2572908682730098No Hit
TCGAGTGGTGCGATGTTCTGGTCTTACAGATGGTCTTCTCGATTGATCTC1732170.23319669061212647No Hit
TTGAGTTATCCGCAGCGCTAAACCCTGTCATCTCCATGACCCTACTTGAC1644640.22141279738612704No Hit
TTGAGTTCATGACCAACAACCCAAAAGCAAATGCAACAAAAACAAAGATA1536720.20688386151328508No Hit
CAAGTGCGATATGGGGCTTTGCAGTTGGTGCGGGTGGCGAAGGCGTTGAA1497950.20166437630396256No Hit
TTGAGTATTTTGCAATTCCGATAATTTGTAAAAGCCAAAAATTAAGAGAC1484720.19988326231584452No Hit
TTGAGTTCGGAAGCTGCGATCGGGCTACTCTAGGAAAGAACACGAATGAA1483800.1997594055608129No Hit
TTGAGTGCTTTGTAAACCGGCTCAACCTTCATCACCATCCCAACTCCCCC1429030.192385889829201No Hit
TTGAGTATCATAATGGATCTCCCAGAAAATGTCTTGTGTCAGTGGGCAAA1428540.19231992264445588No Hit
TTGAGTTATCCGCTATCTCCCTGCACTCATCTTGCCCCCAGCTTGGATTC1426120.1919941255279596No Hit
TTGAGTATCGAGCGTTATCCATTTTGAAATTCTTTTCCGCCGTATACTTT1424270.19174506574881992No Hit
TTGAGTATCATGTTGAATAAGCTTCTGATATCAAAATATTTATCAAAAAA1390620.18721487030663003No Hit
TTGAGTACTAACGAATCGAATGGAATGGAATGGATTCTAATTGAAAGGAA1382860.18617016550331966No Hit
TTGAGTATCATGTTTCTTGGCCATTTGCATATCTTCTTTTGAGAATTGTC1350550.1818203701173715No Hit
TTGAGTGAATAAGATATGATTAAGTTGCATATAGATGCTTTGGCGAAATT1348230.18150803569163954No Hit
TTAAGAAAAGATACGAAAGTTTATTCCAAGAATATAAAGCTAGATATGAT1319530.17764424344228294No Hit
TTGAGTTGACCCGATCAAGTGCTCCTTACCACGTAAAACACATCAAAGAA1279040.17219319995181434No Hit
CAAGCAATTTAACCAGCACCGCCCCAACCTCAGGCACAATCGGCTACTGG1269540.17091424432920502No Hit
GCAAGTGCGATATGGGGCTTTGCAGTTGGTGCGGGTGGCGAAGGCGTTGA1250090.16829575097554694No Hit
TTGAGCAGCACAAGCAAGCAGCTCAGCTGGAGTACATGTCCCACCAGTCG1232270.16589669948134714No Hit
TCAAGCAATTTAACCAGCACCGCCCCAACCTCAGGCACAATCGGCTACTG1231440.1657849591480034No Hit
TTGAGTAGACACTTTTTCCTGTCTTGACTGCTGCCGTGAAACCCGATCCT1218240.16400788396711463No Hit
TTGAGTTATCCGCTCATGCATACATAAGAAGACTTCAAGGAGCTCTTCTG1209420.1628204746417026No Hit
TTGAGTCCATTCCATTCCATTTCCTTCAATTCTATTCCATTGGCGTCCAT1185720.15962981693056144No Hit
TTGAGTTCCAATGACTTGAAGATAAAAAAGAAGAAAAAGTTAAAGAAGTA1179670.15881532413932076No Hit
TTGAGTAGACACTGATAGCATTCACCTGAGCTTCGTGAAGTTCGATTTCC1175860.15830239562120058No Hit
TGAGTTCAACTACAGTAAGTACTAGTTTATGTAGGTATTCCACTGAAAGT1146990.15441571679754468No Hit
TTGAGTTCATCTGTGGTTAACTGAGAGAGAATTTCTCACCCAAAATAATT1131760.15236534899414045No Hit
TTGAGTAGACACTTTTGGGAACTTTCGCCGTATTAGTTTCAGGTGCAGTA1128880.15197762350012833No Hit
TTGAGTATCATGTTTTATGGATGAATATAGATGTGATTGAATGTGATTGA1123250.15121967401009775No Hit
TGGAGAAGTCTTATAACTCAGTTGAGATTTGTTGGACAGTAGCTTTGTGA1109750.1494022107569161No Hit
TTGAGTGAATACGAAGAACAAATGAAACCATTCTACTTAATGCTTCTGCC1094730.14738011460411693No Hit
TTGAGTAAAACAACATCCCATTTTTCCAAATCACTAATATCGATATGTGC1091500.14694526969242977No Hit
TTGAGTTCGGAAGTTGGCAGACGGTCTTACATTACGGCTGCGCAATTAGA1091230.1469089204273661No Hit
TTGAGTAGACACTCCTGCTAATGTGATGCGAGCCTAGCGCTCGCTTGCAT1081630.1456165021139925No Hit
TTGAGTTATAGCAAACGGTGTATGGTTTTTTGAGGAGGTCATATTGTTGT1070530.14412214343915422No Hit
TTGAGGTAAAGTGATAAAAGTCGACCTGGAGAAGTCTTATAACTCAGTTG1059300.14261028326632233No Hit
TTGAGCATCATCAAAAATAATCGCTATACCGCTGCTACGTAGCTTCTCCT1057840.14241372798116345No Hit
TTGAGTTCGGAAGAGACCCCTCCAAAGTCGCCACTAGTACACTGCCCGGC1049890.1413434440654009No Hit
TTGAGTAGACACTGGGGACGCTGCGAAGGACAAGGAAATGGAATTCCAGT1045100.1406985811777905No Hit
TTGAGGAATCCTCCTGCCACGGCCTCCCAAAGTACTGGGATTGTAGGGGG1030820.1387761089366472No Hit
TTGAGTTGTGCGGCGGGGATACTTCACCACCAATCAGCTTCATCAACACC1021940.1375806219967766No Hit
TCCAGACTCTGCGGTAAAGGAAAGCTCATTCTGTAATGGAAAGAAGCTGG988500.13307869820519178No Hit
TTGAGTATGATGGGGTGGTGGTTGTGGTAAACTTTAATAGTGTAGGAAGC988120.13302753998028738No Hit
TGGAGGCAAACCATCACATTGCCAGTCTCCCTTGCAACTCTGGTTATGAA976360.13144432754640467No Hit
TTGAGTCACCACGACAAGAAAGCATCGAATCACCAGGAACACACACCAGA968150.13033904063465493No Hit
TTGAGTTTGAACTTGATGCTGACGAGGTGGTGAGTTACAAAAGAGACTAT957380.12888910883933888No Hit
TTGAGTTTCATGCTTTCTTCTAAACTCTTTTTTTCTTCCTGCGCTCTTTT953500.1283667564376837No Hit
TTGAGTATCATGTCCCCCGCACCAATAGGATCCTCCCGAATCAACCCTGA942660.12690740075883264No Hit
TTGAGTTACACGTTCAACCGGTGTCTCACGTCCAAAGAGTGAAACAAGTA898100.12090842575425667No Hit
TTGAGTAACACGCCTCGTGTAACTGGATTCGTTGGTGCTGGTGTAAATCC894080.12036722558553146No Hit
TGAGTAACAACACCAACTTTTTCGATATCTGGACGTGATTTTAATTCTGC888800.11965639551317596No Hit
TTAAGTGTCAGTGGTGGACTGACCGTTGGCGGCGATACTAACTTTAACGG886910.11940195065773052No Hit
TTGAGTATCGGAACTTAATTAGAATCTGAAGGATTTGATGCAGCAGAAAT881320.11864938624400567No Hit
TTGAGTATGCTTCTTCCATGTTCTTTCCTGCTTTCCTTGCTGTTCCCGTT873050.11753602171779734No Hit
TTGAGTAGACACTCCTTCGCACTGACTAAATACCCTGTAGATTCTCCAGA864280.11635534373776746No Hit
TTAAGTTAAGCGCTACGCTTTCACTGCCAACTTTAAGATTACTCAAATCA830260.11177533633974963No Hit
TCAAGACCAACAAGAATCGAACGGCGGCACAGAAACAAACGATGGGTGCT817010.10999152981347872No Hit
TTGAGTGTAATCGAATGGACTCATCCAATGGAATCAAATGGAATCATCAA810430.10910568476118722No Hit
TTGAGTCCATTCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT807780.10874892345593304No Hit
TTGAGTCTGTGAGCGTATGGACCACATTGGAAGTTCGGCATTGACAAGAC794400.10694761543166853No Hit
TTGAGTATCGGAACCCTAATGACCCCCGCCGAGCAACTACAAGAGATCAT789340.1062664032789945No Hit
TGAGCAGCACAAGCAAGCAGCTCAGCTGGAGTACATGTCCCACCAGTCGA775660.10442470718243707No Hit
TGAGTTCGGAAGATTGGGCGACCAAAGGGGTGGTAAATGTTACTGTCATA757700.10200680792116722No Hit
CAAGGAAGAATTGCGACATCAAGTCGGAGAGATTGATAGCGTCGAACCAG755030.10164735407776018No Hit
TTGAGTAGACACTTTGATACTGTTAAGGTTCCGGCGGAAGTAATTGTGCC751710.10120039274438514No Hit
TCAAGACTCCGGGTTCGTAAGCTTGAATTTTCGATGGGTGTTTTTCCAAC751670.1011950076680794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA12249350.0137.730271
TTGAGTT13853250.0131.129461
TTGAGTC5766800.0129.308241
TTGAGTG6098800.0114.787841
CGCCTTA694850.0107.5332643
GTGAGTA1470450.0105.7245941
TGAGTAC4997850.0105.558932
GAGTACT4093000.0103.5493
GCCTTAC738950.0101.41994
GCCGTAA1218200.0100.096167
TGAGTAT5691800.099.9941942
TGAGTTA9729050.099.250312
GAGTGCC1526600.097.491133
TGAGTTC6677800.096.835862
CCTTACA772850.096.764885
GAGTATC3662050.096.334683
CCGCCTT778700.095.861032
GAGTTAT2783900.095.788563
AGTTACC2217450.095.4483954
ACTAACG1893100.094.1066747
Produced by FastQC (version 0.11.2)