FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16751633
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC222350.13273332814776923No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG204370.12200004620445064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA91500.017.51759768
TGCGCAT97900.016.44411769
GGTGCGC101000.016.216767
AGGTGCG109700.014.77222966
CGGGGGT14150.014.3519381
GCTGACT49650.012.905381
AGGGGGG11250.012.7537974
CCGGGGT13050.012.6103271
CTAGCAG54350.012.2328378
AAGGTGC139250.011.91410265
CGAGCGC60750.011.75838159
CGGGAAT110850.011.3395991
CGGGCAT21850.011.2172281
ACTCTAG70750.011.1292735
AGCGCGC63650.011.05701661
GACTCTA72800.011.0561674
GCGCGCC66750.010.648066562
TCTAGCA117150.010.5737157
ACGAGCG68750.010.54308658
CCGGGAT42450.010.4752651
Produced by FastQC (version 0.11.2)