FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16548132
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC240310.14521880777842477No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG190730.11525772214048087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT12550.015.345131
GGGGCAC10500.015.0063421
GTGCGCA92700.014.64873568
TGCGCAT97900.013.97807769
CTAGCAG51350.013.9690568
GGTGCGC104750.013.53184467
GCTGACT55750.012.812591
AGGTGCG107850.012.7871666
CGGGGAG29550.012.6787931
CGCGGGG13900.012.0914411
CTGTACG158350.011.7129226
TCTAGCA110900.011.6425257
CGGCAAT36750.011.5286821
GACTCTA75900.011.5266134
CGGGCAT23800.011.4754381
TGTACGA164000.011.414647
ACTCTAG74000.011.3970245
AGGGGGG8650.011.3277974
GGGATCT184700.011.2987651
GCGCTTC165250.011.07664868
Produced by FastQC (version 0.11.2)