FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11882253
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC169520.142666546487438No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG150750.12686987896992263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA64800.015.82451568
CCGGGGT9350.015.7289721
TGCGCAT67450.015.15097269
GGTGCGC72000.014.53407767
CTAGCAG39450.013.9258278
GCTGACT40700.013.7653821
GACTCTA52150.013.6892854
AGGTGCG76500.013.634366
ACTCTAG53500.012.6244385
GGGGCAC7550.012.5221671
CGGGCGC11900.012.3507315
CGGCAAT24350.012.0793351
TCTAGCA83950.011.9624587
CGGGACT19250.011.823481
CGAGCGC42350.011.57514259
TCGGGCG15650.011.4028947
CGGGGGT11600.011.16879751
ATCGGGC17700.011.071936
CTGACTC52350.010.9638062
CGCGGGG9750.010.7740581
Produced by FastQC (version 0.11.2)