FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11455510
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC164720.14379106648241763No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG153790.1342498064250304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA69150.017.20796468
TGCGCAT72350.016.59241969
GGTGCGC78200.015.48557367
CCGGGGT7000.015.0063321
AGGTGCG81100.014.58760866
CGGCAAT21800.013.8131691
CTAGCAG35100.013.4585228
TAGCCCG12400.013.26356234
CGGGCGC12150.012.9605115
GGGGCAC5651.8189894E-1212.3946111
GACTCTA49750.012.3102494
TCGGGCG15800.012.1807087
CGGGGGT11050.012.0412811
AGGGGGG6200.011.85357954
ACTCTAG48750.011.7731595
AAGGTGC102950.011.72959165
CGGGACT16250.011.6356781
CGGGCAT15950.011.6350041
GGCGCAT14600.011.50467217
CGCGGGG7650.011.4427371
Produced by FastQC (version 0.11.2)