FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9243229
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC131650.14242858204638226No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG117170.1267630608307984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA49850.017.05983268
TGCGCAT53750.015.82208869
GGTGCGC55700.015.58250167
AGGTGCG58050.014.77201766
CCGGGGT6750.014.0069241
CGCGGGG8350.013.41980651
CTAGCAG28600.012.7240628
CGGCAAT18550.012.6477611
GCTGACT28750.012.5453321
CGAGCGC32750.012.296020559
CCCTACA10150.012.06605110
CGGGGGT9650.011.9748321
CGGGCAT12000.011.6724371
ACTCTAG39100.011.6361595
GCGGGGC5451.382432E-1011.5591552
GGGCGCT3702.0227308E-611.3569661
AAGGTGC78050.011.34549765
GCGCGCC36350.011.26970462
AGCGCGC35850.011.23221961
CGGGGGG12150.010.9519161
Produced by FastQC (version 0.11.2)